Potential Mechanism of Immune Enhancement of Shouzheng Drink as Tea Based on Network Pharmacology and Animal Ex-periment
Objective:Network pharmacology and in vivo experiments were used to investigate the potential mechanism of Shouzheng drink as tea to immune enhancement in normal mice.Methods:By obtaining the active ingredients and corresponding target of TCM-SP,Swiss Target Prediction databases,established the data sets;GeneCards database obtains immune-related targets and intersects with drug targets;PPI network analysis and GO and KEGG enrichment analysis of the intersection targets by STRING and DAVID data-bases,The network map of"Shouzheng drink as tea-potential active compound-target"and"component-target-pathway"network map were constructed by Cytoscape 3.9.1,molecular docking validation using AutoDock Tools.Mice carbon clearing experiment,delayed type hypersensitivity experiment and serum hemolysin experiment and Western Blot to verify some targets.Results:A total of 108 ac-tive ingredients of Shouzheng drink as tea were obtained,and 114 intersection targets of the immunity enhancement,The core ingredi-ents of Shouzheng drink as tea in the enhancement of immunity included quercetin,beta-sitosterol,kaempferol,stigmasterol,luteolin;the core targets included PTGS2,HSP90AB1,PRKACA,RELA,Akt1,it is mainly involved in the response to drugs,positive regula-tion of gene expression,and negative regulation of the apoptosis process,TNF,PI3K-Akt and IL-17;key targets and core components have a strong affinity.Results of the animal experiments are shown,compared with the blank group,the low,medium and high doses groups of Shouzheng drink as tea enhanced the level of delayed allergic reaction in mice,and the medium and high doses groups could improve the phagocytic index of macrophage,the protein expression of p-PI3K,p-Akt and p-P65 were up regulated in the medium and high-dose Shouzheng drink as tea groups(P<0.01).Conclusion:Shouzheng drink as tea has the function of enhancing immunity,perhaps through PI3K-Akt/NF-κB signaling pathway promotes the release of immune active substances and exerts immune regulatory effects.
Shouzheng Drink as TeaImmune enhancementNetwork pharmacologyMolecular dockingPI3K-Akt/NF-κB pathway
万方数据
维普
