长江流域资源与环境2024，Vol.33Issue(2) ：398-408.DOI:10.11870/cjlyzyyhj202402014

嘉陵江河岸湿地土壤好氧甲烷氧化潜力及关键功能微生物研究

Aerobic Methane Oxidation Potential and Key Methane Microbial Oxidizers in the Jialing River Riparian Wetland Soils

杨蕊毓 彭超 叶雨秋 莫永亮 路璐
长江流域资源与环境2024，Vol.33Issue(2) ：398-408.DOI:10.11870/cjlyzyyhj202402014
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嘉陵江河岸湿地土壤好氧甲烷氧化潜力及关键功能微生物研究

Aerobic Methane Oxidation Potential and Key Methane Microbial Oxidizers in the Jialing River Riparian Wetland Soils

杨蕊毓 1彭超 1叶雨秋 1莫永亮 2路璐2
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作者信息

  • 1. 西华师范大学生命科学学院,四川南充 637002
  • 2. 西华师范大学环境科学与工程学院,四川南充 637002
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摘要

河岸湿地是水域和陆地生态系统的交错带,也是微生物甲烷产生和氧化的热点区域.以嘉陵江(南充段)河岸湿地土壤为研究对象,采用室内微宇宙CH4氧化培养实验,以及基于13CH4的稳定性同位素核酸探针(DNA-Stable Isotope Probing,DNA-SIP)技术和高通量测序技术,揭示该土壤中微生物的甲烷氧化潜力及其活性的好氧甲烷氧化微生物类群.结果表明,土壤中加入6％(v/v)的甲烷培养28 d后,甲烷的平均氧化速率为11.94 μg g-1 d-1.通过对超高速密度梯度离心获得的DNA中pmoA基因的定量分析表明,好氧甲烷氧化微生物的DNA被13C显著标记.对获得的13C-标记的DNA测序发现,Type Ⅰ和Type Ⅱ的甲烷氧化菌主导了该土壤中的好氧甲烷氧化过程,其中Type Ⅰ的相对丰度最高78.49％,包括Methylomicrobium、Crenothrix、Methylogaea,其中Methylomicrobium占比高达61.37％;隶属于Type Ⅱ的Methylocystis参与了该土壤的好氧甲烷氧化过程.此外,FAPROTAX功能注释预测结果显示,13C-DNA中微生物参与与碳循环相关的化能异养、甲醇氧化、甲基营养代谢等碳循环的功能,以及氮循环相关的固氮功能均显著增强,表明甲烷氧化微生物在进行甲烷氧化的同时,可能协同参与氮循环等其它生物地球化学循环过程.研究表明河岸湿地土壤的好氧甲烷氧化过程由多种具有代谢活性的微生物共同完成,为研究河岸湿地甲烷氧化关键微生物的生理生态特征提供参考.

Abstract

Riparian wetland,the transitional zone of river and terrestrial ecosystems,is a potential hotspot for microbial methane production and oxidation.Here,a 13CH4-based stable isotope nucleic acid probe(DNA-SIP)microcosm incubation experiment combined with high-throughput sequencing and chemical analysis was employed to reveal the methane oxidation potential and active aerobic methane-oxidizing microorganisms in sediment collected from a riparian wetland soil of the Jialing River(Nanchong section).The results showed that the soil methane oxidation rate was 11.94 μg g-1 d-1 after incubation with 6％(v/v)CH4.Analysis of the pmoA gene distribution in DNA obtained by ultra-high speed density gradient centrifugation showed that the DNA of the aerobic methane-oxidizing bacteria was significantly labeled with 13C.Sequencing of the 13C-labelled DNA revealed that methane-oxidizing bacteria(MOB)belonging to Type Ⅰ,and Type Ⅱ dominated the aerobic methane oxidation process.Type Ⅰ MOB accounted for 78.49％of the labelled MOB,which including Methylomicrobium,Methylogaea,and Crenothrix.Methylomicrobium accounted for 61.37％of the labelled Type Ⅰ MOB.Methylocystis affiliating to Type Ⅱ MOB was the main responder in methane oxidation in the soil.In addition,FAPROTAX functional annotation of 13C-DNA showed that the function of microbial communities involved in the carbon and nitrogen cycles were stimulated,such as chemoheterotrophy,methanol oxidation,methyl compound metabolism,and nitrogen fixation.This result suggested that microbial methane oxidation could be correlated with other biogeochemical cycling processes.This study highlighted that the aerobic methane oxidation in the riparian wetland soil was performed by a variety of metabolically flexible microorganisms,and provided basic data for physiological ecology study of methanotrophs in riparian wetlands.

关键词

河岸湿地/甲烷氧化/稳定性同位素核酸标记/甲烷氧化微生物/碳氮循环功能

Key words

riparian wetlands/methane oxidation/stable isotope nucleic acid markers/methane oxidizing mi-croorganisms/carbon and nitrogen cycle function

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基金项目

四川省科技厅应用基础研究和重大研发项目(2022YFN0065)

国家自然科学基金项目(42107278)

西华师范大学科研创新团队基金项目(CXTD2020-2)

出版年

2024
长江流域资源与环境
中国科学院资源环境科学与技术局 中国科学院武汉文献情报中心

长江流域资源与环境

CSTPCDCSSCICHSSCD北大核心
影响因子：1.35
ISSN：1004-8227
参考文献量8
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