Promotion of the proliferation of hepatocellular carcinoma cells by acetyl-CoA carboxylase 2 through cell cycle regulation
Objective:To investigate the impact of acetyl-CoA carboxylase 2(ACC2)on the proliferation and migration of hepatocellu-lar carcinoma cells(HCCs)as well as the potential mechanism of action.Methods:The expression of ACC2 in the liver tissue of pa-tients with HCC and its prognostic significance were analyzed in The Cancer Genome Atlas,Gene Expression Omnibus,and Clinical Proteomic Tumor Analysis Consortium databases.The ACC2-knockdown cell lines of HCCs were established using the CRISPR-Cas9 system;the changes in proliferation,migration,and cell cycle of the HCCs were observed.The expression of cell cycle-related proteins was measured using Western blot assay.Results:ACC2 showed low expression in the HCC tissue(P＜0.05)and was associated with un-favorable prognoses(P＜0.05).In vitro cell functional experiments revealed that the decrease in ACC2 expression significantly en-hanced the proliferation and migration of the HCCs(P＜0.05).Flow cytometric analysis of cell cycles proved that ACC2 knockdown pro-moted the Gl/S phase transition(P＜0.05),with decreased expression of CyclinE2 and p21 and increased expression of CyclinA2 and p-RB among the cell cycle-related proteins.Conclusion:ACC2 promotes the proliferation and migration of HCCs,which is achieved by accelerating the G1-to-S phase transition of the HCCs.
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