Molecular mechanism of Buzhong Yiqi Decoction in coordination with Siβ-catenin in intervention against cisplatin resistance in lung adenocarcinoma by regulating the Wnt/β-catenin signaling pathway
Objective:To investigate the molecular mechanism of Buzhong Yiqi Decoction in coordination with Siβ-catenin in inter-vention against cisplatin resistance in A549/DDP cells by regulating the Wnt/β-catenin signaling pathway.Methods:The expression ofβ-catenin was silenced by siRNA interference,and Western blot was used to measure the relative protein expression of β-catenin in A549/DDP cells in the normal serum group,the cisplatin group,the siCTNNB1-235 group,the siCTNNB1-510 group,and the siCTNNB1-547 group.After β-catenin interference,Western blot was used to measure the protein expression levels of β-catenin and Survivin in A549/DDP cells of each group;MTT assay was used to determine the IC50 of cisplatin in A549/DDP cells of each group;Annexin V/PI staining was used to measure the cisplatin-induced apoptosis rate of A549/DDP cells in each group.Results:Compared with the normal serum group(1.00),the siCTNBN1-235 group(0.323±0.021)had an inhibition rate of 67％on β-catenin expression(P=0.000).After β-catenin was silenced by RNAi technique,compared with the normal serum group(1.00),the Buzhong Yiqi Decoc-tion+cisplatin group had significantly greater reductions in the protein expression of Survivin(0.247±0.015)and β-catenin(0.257± 0.015)(P=0.000,P=0.000).As for the IC50 of cisplatin in A549/DDP cells,compared with the normal serum group(28.330±1.029)μmol/L,serum containing Buzhong Yiqi decoction alone(20.350± 1.155)μmol/L or siCTNNB1-235 transient transfection alone(15.577±1.535)μmol/L reduced the IC50 of cisplatin in A549/DDP cells(P=0.000,P=0.000),and the combination of the two methods(10.453±0.999)μmol/L further reduced the IC50 of cisplatin(P=0.000).Compared with the normal serum group(9.130±0.384)％,the siCTNBN1-235 transient transfection+cisplatin group(64.393±0.244)％and the Buzhong Yiqi Decoction+cisplatin group(70.120±0.400)％had a significant increase in overall apoptosis rate(P=0.000,P=0.000).Conclusion:Serum containing Buzhong Yiqi Decoction and siβ-catenin transient transfection have asynergistic effect on inhibiting the Wnt/β-catenin signaling pathway and improving cisplatin resistance in lung adenocarcinoma,and Buzhong Yiqi Decoction has a siβ-catenin transient transfection-like effect.
β-cateninSurvivinRNAilung cancer drug resistanceBuzhong Yiqi Decoction
万方数据
维普
