Proteasome activator subunit 1/2 promotes the proliferation and invasion of endometrial carcinoma cells by inhibiting the expression of cyclin-dependent kinase 15
Objective:To investigate the expression level of proteasome activator subunit 1/2(PSME1/2)in endometrial carcinoma and its effect on the proliferation and invasion of endometrial carcinoma cells.Methods:The expression levels of PSME1/2 and cyclin-dependent kinase 15(CDK15)were measured in endometrial cell lines and primary cultured cells,and knock-down strains were used to analyze the correlation between PSME1/2 and CDK15 and the effect of PSME1/2 on tumor proliferation and invasion.The expression levels of PSME1/2 and CDK15 were compared between endometrial carcinoma tissue and paracancerous tissue,and the influence of PSME1/2 and CDK15 on the prognosis of patients with endometrial carcinoma was analyzed.Results:Compared with normal human en-dometrial cell lines,human endometrial adenocarcinoma HEC1B cells and primary cultured cells had high mRNA and protein expres-sion levels of PMSE1/2 and a reduction in the protein expression of CDK15.Compared with the control group and the double knock-down group,there was an increase in the protein expression level of CDK15 in the HEC1B line with PSME1/2 knock-down,suggesting that CDK15 might be inhibited by PSME1/2.There was a high expression level of PMSE1/2 and a low expression level of CDK15 in en-dometrial carcinoma.There were no significant differences in age,postoperative diagnosis and typing,degree of tumor differentiation,and postoperative stage between the high PSME1/2 expression group and the low PSME1/2 expression group(P＞0.05).There was no significant correlation between the expression of PSME1/2 and CDK15 and the poor prognosis of patients with endometrial carcinoma(P＞0.05).Conclusion:PSME1/2 can inhibit the expression of CDK15 and promote the proliferation and invasion of endometrial carcinoma cells,and PSME 1/2 can also promote endometrial carcinoma.
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