Impacts of LncRNA MYLK-AS1 on proliferation,apoptosis,and invasion of gastric cancer cells by regulating the miR-141-3p/STMN1 axis
Objective:To investigate the impacts of long non-coding RNA(LncRNA)myosin light chain kinase antisense RNA1(MYLK-AS1)on proliferation,apoptosis,and invasion of gastric cancer cells by regulating the miR-141-3p/stathmin 1(STMN1)axis.Methods:HGC27 cells were grouped into negative control(NC)group,si-NC group,si-MYLK-AS1 group,si-MYLK-AS1+in-hibitor NC group,and si-MYLK-AS1+miR-14.1-3p inhibitor group.The relationship among LncRNA MYLK-AS1,miR-141-3p,and STMN1 was determined by the dual-luciferase reporter gene assay;the expression of LncRNA MYLK-AS1 and miR-141-3p in HGC27 cells was measured by qRT-PCR;the CCK-8 method and flow cytometry were usde to determined the proliferation and apopto-sis of HGC27 cells,respectively;the numbers of invading and migrating HGC27 cells were counted by Transwell;the protein levels of STMN1,E-cadherin,Vimentin,and N-cadherin in HGC27 cells were measured by Western blot.Results:Compared with GES-1 cells,the levels of LncRNA MYLK-AS1 and STMN1 in HGC27 cells were significantly up-regulated(P＜0.05),and the level of miR-141-3p was significantly down-regulated(P＜0.05).Compared with the NC group and si-NC group,the si-MYLK-AS1 group showed significant decreases in the optical density at 450 nm,numbers of migrating and invading cells,expression level of LncRNA MYLK-AS1,and protein levels of STMN1,N-cadherin,and Vimentin(P＜0.05),as well as significant increases in the apoptosis rate,expres-sion level of miR-141-3p,and protein level of E-cadherin(P＜0.05).However,the low expression of miR-141-3p attenuated the inhi-bition of HGC27 cell development by silencing LncRNA MYLK-AS1;LncRNA MYLK-AS1 regulated the miR-141-3p/STMN1 axis in a targeted manner.Conclusion:Silencing LncRNA MYLK-AS1 may inhibit the expression of STMN1 by up-regulating miR-141-3p,thus affecting the proliferation,apoptosis,and invasion of gastric cancer cells.
万方数据
维普
