Objective:To select genes associated with disulfidptosis-related myocardial ischemia-reperfusion injury(MIRI),and to explore their possible pathways of action.Methods:We selected differentially expressed genes associated with MIRI between the 24 h group and the control group with the use of the limma R package;performed functional enrichment analysis on the genes through the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases with the use of the clusterProfiler R package;conducted en-richment analysis based on disulfidptosis-related gene set and GSE160516 expression data using the ssgsea method of the GSVA R package,and identified the expression of disulfidptosis-related genes in myocardial ischemia-reperfusion using a Venn diagram;calcu-lated the correlations between disulfidptosis-related genes and genes associated with apoptosis factors,mitochondria,ferroptosis,and inflammation using the corr.test of the psych R package,and generated a heatmap;and clustered the expression patterns of MIRI tran-scriptome data at different time points using the Mfuzz R package,and identified time series-related differentially expressed disulfidptosis-related genes using a Venn diagram.Results:A total of 17 differentially expressed disulfidptosis-related genes were determined in this study.Through correlation analysis of disulfidptosis-related genes and genes associated with inflamma-tion,apoptosis,ferroptosis,and mitochondria as well as analysis of time series-related differentially expressed genes associated with di-sulfidptosis during myocardial ischemia-reperfusion,we finally identified the specific expression of disulfidptosis-related Flna,Myl6,and Tln1 genes at different time points pf MIRI.Conclusion:The Flna,Myl6,and Tln1 gens may play crucial roles in MIRI,and these disulfidptosis-related genes are closely associated with mitochondria-related genes,which can be screening indicators for risk factors in patients with MIRI.
维普
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