Lipid metabolism-related gene ABHD5 inhibits renal cancer cell migration and invasion in vitro
Objective:To investigated the relationship between clear cell renal cell carcinoma(ccRcc)cells and α/β-hydrolase domain-containing 5(ABHD5)during migration and invasion in vitro.Methods:The TCGA database was used to analyze the expres-sion of ABHD5 in human ccRcc tissues and normal kidney tissues.After transfection of human ccRcc cell lines 786-O and Caki-1 with ABHD5-overexpressing lentivirus,the changes in ABHD5 expression were detected using real-time PCR and Western blot.Tran-swell assay and scratch assay were used to examine the effects of ABHD5 on cell migration and invasion in vitro.Results:TCGA data-base showed that the expression of ABHD5 in ccRcc tissues was significantly decreased compared with normal renal tissues(P=0.000),and the decrease was greater in recurrent metastatic tumors(P=0.007),which was significantly negatively correlated with the survival of the patients(P＜0.001).Transwell assay and scratch assay showed that ABHD5 overexpression significantly inhibited the migration and invasion of ccRcc cells in vitro(P＜0.05).Overexpression of ABHD5 inhibited renal cancer cell migration and invasion in vitro by influencing the Sting signaling pathway.Conclusion:Lipid metabolism-related gene ABHD5 inhibited the migration and in-vasion of ccRcc cells in vitro.
万方数据
维普
