Adenosine deaminase acting on RNA 1 p150 and p110 are involved in the proliferation,migration,and invasion of liver cancer cells
Objective:To investigate the effect of adenosine deaminase acting on RNA 1(ADAR1)on the proliferation,migration,and invasion of liver hepatocellular carcinoma(LIHC)cells.Methods:The gene expression profiling interactive analysis website GEPIA was used to obtain the expression level and prognostic value of ADAR1 in LIHC;proteins were extracted from human hepatocellular carcinoma specimens,and Western blot was used to measure the expression level of ADAR1.HepG2 and Huh7 cells were transfected with ADAR1 p150 and p110 overexpression plasmids or small interfering RNAs targeting both ADAR1 p150 and p110,and Western blot and qPCR were used to measure the expression of ADAR1 in cells after transfection.CCK-8 assay was used to observe the effect of ADAR 1 on the proliferative ability of hepatocellular carcinoma cells,and scratch assay and Transwell assay were used to observe the effect of ADAR1 on the migration and invasion of LIHC cells.Finally,the DAVID online database was used to analyze the signaling pathways related to ADAR1,and Western blot was used to measure the expression of proteins associated with the signaling pathways.Results:The protein expression level of ADAR1 in hepatocellular carcinoma tissue was significantly higher than that in paracancerous tissue,which was consistent with the prediction based on bioinfor-matics,and the high expression of ADAR1 was associated with the poor prognosis of hepatocellular carcinoma.CCK-8 assay showed that overexpression of ADAR1 promoted the proliferation ability of hepatocellular carcinoma cells(P＜0.05),and knockdown of ADAR1 reduced the proliferation ability of hepatocellular carcinoma cells(P＜0.05).Scratch assay and Transwell assay showed that overexpression of ADAR1 significantly promoted the migration and invasion of hepatocellular carcinoma cells(P＜0.05),and knock-down of ADAR 1 inhibited the migration and invasion abilities of hepatocellular carcinoma cells(P＜0.05).The results of DAVID online analysis showed that ADAR1 was mainly enriched in the Wnt signaling pathway.Western blot showed that overexpression of ADAR1 inhibited the expression of GSK3β and the phosphorylation level of β-catenin,and knockdown of ADAR1 increased the expression of GSK3β and the phosphorylation level of β-catenin.Conclusion:The results of this study show that there is a high expression level of ADAR1 in LIHC,which can activate the Wnt/β-catenin signaling pathway and thus promote the proliferation,migration,and invasion of hepatocellular carcinoma.
adenosine deaminase acting on RNA1hepatocellular carcinomaproliferationmigration and invasionWnt/β-catenin signaling pathway
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维普
