Impact of florfenicol on decidualization of endometrial stromal cells
Objective:To investigate the impact of florfenicol(FLO)exposure on decidualization and the role of mitochondria in this process.Methods:Primary mouse decidual stromal cells(mDSCs)were used to establish a model of FLO exposure.After exposure to FLO(1.56,6.25,and 25.00 μg/mL),Phalloidin staining was used to observe cell morphology;Western blot was used to measure the ex-pression of HOXA10,a maker molecule for decidualization;and investigate the influence of FLO on cell proliferation and apoptosis during decidualization;the fluorescent probe Mito—tracker Red was used to observe mitochondrial morphology;immunofluorescence as-say was used to measure the expression of TOMM20,a marker protein for outer mitochondrial membrane,and the level of ATP to ob-serve the influence of FLO on mitochondrial function in mDSCs.Results:Exposure to 25.00 μg/mL FLO led to abnormal cell morphol-ogy,reduced the expression of the marker molecule for decidualization HOXA10(P=0.002),promoted apoptosis,and inhibited cell pro-liferation.Further analysis showed a reduction in the expression of marker protein for outer mitochondrial membrane TOMM20(P=0.010),abnormal mitochondrial morphology,and a reduction in the level of ATP(P=0.003).Conclusion:Exposure to 25.00 μg/mL FLO may disrupt the balance between the proliferation and apoptosis of mDSCs and damage the decidualization process,which is asso-ciated with mitochondrial function.
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