重症肺炎支原体肺炎患儿外周血长链非编码核糖核酸P50相关的环氧合酶2外源性核糖核酸与核因子-κB表达的相关性及临床意义
Correlation and clinical significance of long non-coding RNA p50-associated cyclooxygenase-2 extragenic RNA and nuclear factor-κB expression in peripheral blood of children with severe Mycoplasma pneumoniae pneumonia
王通敏1
作者信息
- 1. 621000,四川绵阳四0四医院(绵阳市第一人民医院)儿科
- 折叠
摘要
目的 探讨重症肺炎支原体肺炎患儿外周血长链非编码核糖核酸(long non-coding RNA,lncRNA)P50相关的环氧合酶2外源性核糖核酸(p50-associated cyclooxygenase-2 extragenic RNA,PACER)与核因子(nuclear factor,NF)-κB表达的相关性及临床意义.方法 收集2020年7月—2023年7月间在绵阳市第一人民医院接受治疗的重症肺炎支原体肺炎患儿238例作为重症组,另选取同期在我院接受治疗的轻症肺炎支原体肺炎患儿100例作为轻症组,体检的健康儿童100例作为对照组.分析3组的外周血lncRNA PACER与NF-κB mRNA表达水平的差异及相关性.根据病情转归将重症组患儿分为好转组(n=197)、恶化组(n=41),对比2组入院后早期外周血lncRNA PACER与NF-κB mRNA表达水平的差异,采用受试者工作特征(receiver operating characteristic,ROC)曲线分析重症组患儿外周血lncRNA PACER、NF-κB mRNA表达量对治疗转归的预测价值.结果 重症组、轻症组、正常对照组的外周血lncRNA PACER、NF-κB表达水平逐渐降低,组间两两比较差异均有统计学意义(P<0.05).重症组患儿外周血lncRNA PACER表达水平与血清NF-κB水平呈正相关(P<0.05).恶化组患儿入院时外周血lncRNA PACER、NF-κB mRNA表达量分别高于好转组患儿,差异均有统计学意义(P<0.05).ROC曲线显示,入院早期外周血lncRNA PACER表达量预测重症肺炎患儿预后不良的最佳截断值为1.715,曲线下面积(area under curve,AUC)为0.816[95％CI:0.733～0.898],对应的灵敏度、特异度分别为71.15％、74.47％;外周血NF-κB mRNA表达量预测重症肺炎患儿预后不良的最佳截断值为2.890,AUC为0.776[95％CI:0.687～0.865],对应的灵敏度、特异度分别为74.0％、63.27％.结论 重症肺炎支原体肺炎患儿外周血lncRNA PACER与NF-κB的表达水平呈正相关且对预后具有预测价值.
Abstract
Objective To investigate the correlation and clinical significance of the expression of lncRNA PACER and NF-κB in peripheral blood of children with severe Mycoplasma pneumoniae pneumonia. Methods Two hundred and thirty-eight children with severe Mycoplasma pneumoniae pneumonia who received treatment in the Mianyang First People's Hospital from July 2020 to July 2023 were collected as the severe group, during the same period, another 100 children with mild Mycoplasma pneumoniae pneumonia who received treatment were selected as the mild group. Additionally, 100 healthy children who underwent physical examination were enrolled in control group. The differences and correlations between lncRNA PACER and NF-κB mRNA expression levels in peripheral blood were analyzed in the 3 groups. The children in the severe group were divided into the improvement group (n = 197) and the deterioration group (n = 41) according to the disease regression. The differences of lncRNA PACER and NF-κB mRNA expression levels in peripheral blood in the early period after admission were compared between the 2 groups. The predictive value of lncRNA PACER and NF-κB mRNA expression in peripheral blood of children in the severe group was analyzed by using the receiver operating characteristic (ROC) curve. Results The expression levels of lncRNA PACER and NF-κB in peripheral blood of severe group, mild group and control group were decreased gradually, and the difference between the pairwise comparison was statistically significant ( P<0.05 ). The level of lncRNA PACER in peripheral blood was positively correlated with the level of NF-κB in serum of children from severe group ( P < 0.05 ). Expression levels of lncRNA PACER and NF-κB mRNA in peripheral blood of children in the deterioration group were higher than those in the improvement group at admission ( P < 0.05 ). ROC curve showed that the optimal cut-off value and AUC of lncRNA PACER expression in peripheral blood in early admission to predict poor prognosis of children in severe group were 1.715 and 0.816 [95％CI: 0.733～0.898], corresponding sensitivity and specificity were 71.15％ and 74.47％ respectively; optimal cut-off value of NF-κB mRNA expression in peripheral blood for predicting poor prognosis was 2.890, AUC was 0.776 [95％CI: 0.687 ～ 0.865], corresponding sensitivity and specificity were 74.0％ and 63.27％ respectively in severe group. Conclusion The expression level of lncRNA PACER and NF-κB in peripheral blood of children with severe Mycoplasma pneumoniae pneumonia is positively correlated and has prognostic value.
关键词
重症肺炎支原体肺炎/lncRNA/PACER/NF-κB/疾病转归Key words
severe Mycoplasma pneumoniae pneumonia/lncRNA PACER/NF-κB/disease outcome引用本文复制引用
出版年
2024
NETL
NSTL
万方数据