Objective To analyze the relationship between gut microbiota and anti-tuberculosis therapy of active pulmonary tuberculosis(ATB)in the northwest China.Methods From July 2020 to October 2023,84 ATB patients(ATB group)and 80 healthy controls(healthy control group)were enrolled in Shaanxi Tuberculosis Prevention Hospital.All ATB patients received routine quadruple anti-tuberculosis therapy(isoniazid+rifampicin+pyrazinamide+ethambutol).Baseline stool samples were collected from all subjects,and additional samples were collected from ATB patients at 6 months of treatment.16S rRNA gene sequencing was used to evaluate the differences in fecal flora between the 2 groups.Biochemical analysis were performed on the patient's blood samples.Results At baseline,compared with the healthy control group,the bacterial group α diversity of ATB patients showed a significant decline trend.The β diversity PCoA analysis showed that the fecal bacterial group composition was different between the two groups,and the fungus group α diversity was also significantly increased.The relative abundance of Bacteroides,Parabacteroides and Veillonella increased in the ATB group compared with the healthy control group,the relative abundance of Faecalibacterium,Bifidobacterium,Agathobacter and CAG-352 decreased significantly.In the composition of fungus group,M.nivalis,Candida,C.albicans were significantly enriched in ATB group,S.cerevisiae,P.kudriavzevii,M.circinelloides,S.coccosporum,A.elegans,A.alternata,H.fuscoatra was significantly decreased(P＜0.05).After treatment,the mean relative abundance of Bacteroides,Bifidobacterium and Ascomycetes in ATB patients gradually increased(P＜0.05).Conclusion The enrichment of gut pro-inflammatory bacteria,the reduction of short chain fatty acid producing bacteria and the imbalance of fungi in ATB patients may cause structural and functional disorders of gut flora.Moreover,antituberculosis treatment can promote the reconstruction of gut microecology in ATB patients in a symbiotic state characterized by fungal enrichment.These results emphasize the importance of gut microbiota imbalance in the process of ATB and anti tuberculosis treatment.
gut microbiotaactive pulmonary tuberculosisbiochemical indexpathological featuresshort-chain fatty acidanti-tuberculosis therapy
维普
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