[Objective]To optimize the current residual detection methods for ractopamine,salbutamol,and clenbuterol in beef to reduce operational difficulties and improve the detection efficiency under the condition of ensuring the accuracy of the results and qualified precision.[Methods]The sample was extracted by 0.1%acetonitrile acetate solution,and defatted by water saturated n-hexane,The extraction solution was concentrated by nitrogen blowing instrument,the re-solubilised solution was detected and analyzed by HPLC-MS/MS,and quantified by internal standard method.[Results]The linear range of this method was 1.0～50.0 ng/mL with good response.The recoveries of ractopamine,clenbuterol and salbutamol at the three spiked levels were 94.3%～107.9%,92.2%～105.7%and 92.2%～102.3%,respectively,and the limits of quantitative detection were lower than 0.06 μg/kg,with the precision of none of them being more than 10%.[Conclusion]The method has a low quantitative detection line,simple step-by-step sample processing,high accuracy and good stability,and can meet the needs of trace detection in large quantities of beef samples.
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