摘要
[目的]利用蛋白组学的方法研究双峰公驼在发情期体重迅速下降但体力仍很旺盛的缘由.[方法]采集公驼发情期和非发情期血液样本为对照,对两个时期的样本进行蛋白表达定量分析,以揭示发情期与非发情期之间蛋白表达的差异.[结果]结果显示,在发情期和非发情期血液样本中,有26种蛋白表达量存在差异,其中在发情期4种蛋白表达量升高,22种蛋白表达量降低;COG分析发现,差异表达蛋白主要涉及细胞分裂、细胞周期、蛋白质周转,翻译后修饰等生物过程;GO分析揭示了蛋白质多种功能的存在,如生物糖原合成、免疫应答、细胞粘着、蛋白聚合、血板活化、信号传递、基微管合成、血液凝固等;KEGG分析发现存在与组织重塑有关的信号通路,以及与重要生物学过程有关的细胞基质黏附通路,如细胞运动、细胞增殖、细胞分化、基因表达调控和细胞存活等,同时还发现了磷脂酰肌醇3′-激酶(PI3K)-Akt信号通路.[结论]研究结果表明,GYS、PPP1R3G、AGP、mTOR、PI3k等蛋白质与公驼发情期低摄食仍能保持充沛精力密切相关.
Abstract
[Objective]A proteomic approach was utilized to study the reasons for the rapid weight lost but still vigorous stamina of Bactrian camel males during estrus.[Methods]Blood samples were collected from male camels during the estrous period,and subsequently blood was collected during the non-estrous period as a control group,and the samples from the two periods were quantitatively analyzed for protein expression to reveal the differences in protein expression between the estrous and non-estrous periods.[Results]The results showed that 26 proteins were differently expressed in the blood samples collected during estrus and non-estrus,of which 4 proteins were increased and 22 proteins were decreased in the blood samples collected during estrus;COG analysis revealed that the differentially expressed proteins were mainly involved in biological processes such as cell division,cell cycle,protein turnover,and post-translational modification;and GO analysis revealed different functions,such as as biosynthesis of glycogen,immune response,cell adhesion,protein polymerisation,activation of blood plate,signalling,basal microtubule synthesis,blood coagulation,etc.;KEGG analysis revealed,among others,signaling pathways related to tissue remodeling,inflammation,and resistance to infectious factors;cell matrix adhesion pathway related to important biological process,such as cell motility,cell proliferation,cell differentiation,regulation of gene expression,and cell survival;and also phosphatidylinositol 3'-kinase(PI3K)-Akt signaling pathway.[Conclusion]The results showed that proteins such as GYS,PPP1R3G,AGP,mTOR,and PI3k were closely related to the ability of male camels to maintain their energetic level even with low food intake during estrus.
基金项目
新疆维吾尔自治区自然科学基金项目(2020D01A40)
国家科技期刊平台
NSTL
万方数据