[Objective]To study the in vitro intestinal absorption characteristics of the main components of milk fat based on the everted intestinal sac method.[Methods]Based on gas chromatography-mass spectrometry(GC-MS),the fatty acid composition of raw milk from a dairy plant was analysed,and then the absorption and transport data of the main components of milk fat in different intestinal segments of rats were obtained by the externally-turned-intestinal-bladder assay,so as to investigate the effect of different intestinal segments,different dosing times and different dosing concentrations on the absorption of the main components of milk fat from the intestinal tract of experimental rats.[Results]A total of 26 fatty acids were analyzed in milk fat,among which 2,3,4-trimethyl-pentanoic acid was the highest(54.68%),followed by 4-methyl-heptanoic acid(22.17%);the optimal administration sites for 2,3,4-trimethyl-pentanoic acid and 4-methyl-heptanoic acid were the jejunum and the duodenum,with the transmitting doses of 31.08×10-3 mg and 20.36×10-3 mg,the absorption conversion rate per unit time was 49.9965%,50.0043%,and the intestinal residual percentage was 99.9931%and 99.9913%,respectively;When the administration time was 120 minutes,the Ka and Papp of 2,3,4-trimethyl-pentanoic acid were 2.63 and 5.82×10-6,respectively,and the Ka and Papp of 4-methyl-heptanoic acid were 4.65and 1.26×10-6,respectively,all of which were the highest;The concentrations of 2,3,4-trimethyl-pentanoic acid in the intestinal vesicle fluid were 9.14×10-3 mg/mL,and 4-methyl-heptanoic acid was 6.36×10-3 mg/mL at a dosage of 10.08 mg/mL,both of them were maximum.[Conclusion]The optimal absorption site for 2,3,4-trimethyl-pentanoic acid was the jejunum,and the optimal absorption site for 4-methyl-heptanoic acid was the duodenum;the optimal time was 120 minutes,and the optimal concentration was 10.08 mg/mL.
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