模拟干旱胁迫下5份野生披碱草属植物萌发期抗旱性评价
Evaluation of Drought Resistance of Five Wild Elymus Plants during Germinatiunder Simulated Drought Stress
韩宇 1梁洑汀 1谷丽丽1
作者信息
- 1. 西部干旱荒漠区草地资源与生态教育部重点实验室/新疆草地资源与生态重点实验室/新疆农业大学草业学院,乌鲁木齐 830052
- 折叠
摘要
[目的]探究5份野生披碱草属种质材料萌发期的抗旱性,以期筛选出抗旱性较好的种质用于进一步种质抗旱研究.[方法]用PEG-6000作为模拟干旱处理试剂,将其配置成0%(蒸馏水)、5%、10%、15%、20%和25%6个浓度梯度的溶液,将不同浓度的溶液加入培养皿中,测定5份材料的发芽势、发芽率、萌发指数、活力指数、根长和芽长.[结果]随着PEG浓度的上升,5份材料的各指标均呈下降趋势,当浓度达到25%时各指标均达到最低值,E02、E04、E05材料已经不能萌发,而E01、E03虽然仍能萌发但萌发能力显著下降.[结论]5%浓度的PEG对材料发芽势和发芽率有一定的促进作用.5份材料的萌发期抗旱性排名为:E01＞E05＞E02＞E03＞E04.
Abstract
[Objective]To investigate the drought resistance of 5 wild Elymus germplasm materials during the germination period,in order to screen out better germplasm for further research on drought resistance.[Methods]With PEG-6000 as the simulated drough treatment reagent,it is configured into 6 concentratio gradient sdutions of 0%(distilled water)、5%、10%、15%、20%and 25%.Different concentrations of solutions were added to culture dishes to determine the germination potential,germination rate,germination index,vitality index,root length and shoot length of the five materials.[Results]As the PEG concentration increased,all the indicators of the five materials showed a downward trend.When the concentration reached 25%,all the indicators reached their lowest values.E02,E04,and E05 materials could no longer germinate,while E01 and E03 could still germinate,but their germination ability was significantly insufficient.[Conclusion]5%concentration of PEG had a certain promoting effect on the germination potential and germination rate of the materials,and the drought resistance of the five materials during the germination period was ranked as follows:E01＞E05＞E02＞E03＞E04.
关键词
披碱草属/PEG胁迫/种子萌发/隶属函数Key words
Elymus genus/PEG stress/seed germination/membership function引用本文复制引用
基金项目
国家现代农业产业技术体系项目(CARS-34)
出版年
2024
国家科技期刊平台
NSTL
万方数据