[Objective]In order to explore the correlation between RXFP2 gene polymorphism and horn traits in Teha mutton sheep.[Methods]a 1.78-kb insertion locus and three SNP loci(rs414104606,rs421908034,rs426516358)of RXFP2 gene were selected according to the relevant literature. PCR combined with agarose gel electrophoresis,and kompetitive allele specific PCR(KASP) technique were used to carry out genotyping of 887 Teha mutton sheep. Among them,425 individuals had horns and 462 individuals had no horns. The genotype frequency and allele frequency distribution were calculated,and the correlation analysis was performed with the angle trait.[Results]The results showed that the four loci (1.78-kb insertion,rs414104606,rs421908034 and rs426516358)were all extremely significantly correlated with the presence or absence of horns. Further analysis showed that 3 loci (1.78-kb insertion,rs414104606 and rs421908034) were all extremely significantly correlated with horn length. The 1.78-kb insertion locus was extremely significantly correlated with the horn base circumference. The rs414104606 locus was significantly correlated with the horn base circumference,and so were the 1.78-kb insertion and rs414104606 locus associated with horn spacing.[Conclusion]1.78-kb insertion was the most ideal molecular marker for breeding hornless Teha mutton sheep.
Teha mutton sheephorn traitKASPRXFP2 genepolymorphism
维普
万方数据
