[Objective]In order to compare the sensitivity,specificity and concordance rate of three methods consisting of i-ELISA、c-ELISA and GICA in detecting brucellosis antibodies.[Methods]For 40 bovine serum samples kept in the laboratory,the positive and negative samples of brucellosis were determined by RBT,c-ELISA as well as SAT method recommended by the national standard. Then the sensitivity,specificity and coincidence rate of i-ELISA,c-ELISA and GICA were compared by detecting Brucella-positive serum samples and the 40 clinical samples.[Results]For the Brucella-positive serum samples,compared with the results of SAT,the specificity and coincidence rate of c-ELISA were 100％,those of GICA 3 were the lowest,91.4％ and 92.1％ respectively. The specificity and coincidence rate of i-ELISA,GICA 1,GICA 2 and GICA 4 were all the same,which were 94.1％ and 94.7％ respectively. For the 40 clinical serum samples,the sensitivity of RBT and 4 kinds of GICA was lower,ranging from 66.7％ to 75％.[Conclusion]The sensitivity,specificity and coincidence rate of c-ELISA were all 100％,while the sensitivity of the 4 kinds of GICA was lower,and there were a certain proportion of false positive and negative samples.
维普
万方数据
