[Objective]Based on the actual situation of the fragile ecological environment of the desert grassland in Hami City,this study aims to analyze the species diversity and niche characteristics of the desert grassland in Hami City,and lay the foundation for the protection and restoration of the desert grassland ecosystem in Hami.[Methods]Vegetation diversity of desert steppe in Hami City as well as the niche characteristics of dominant species was calculated by field investigation in combination with theoretical research method of niche.[Results]There were 45 species of 33 genera of 15 families plants in the desert steppe,mainly Chenopodium. No significant difference was observed in plant diversity between south and north slopes of east Tianshan Mountains.The niche width of dominant vegetation in the desert steppe in Hami City ranged from 0.006 to 1.283,and there were 25 species pairs with overlapping niches,among which,Ephedra przewalskii Stapf and Sarcozygium xanthoxylon Bunge had the highest overlapping value.[Conclusion]Chenopodium was the dominant species of desert steppe in Hami City,and the difference of climate between north and south Tianshan Mountains had no significant effect on vegetation diversity. The niche width of dominant vegetation and overlapping value between species were not high,indicating that the demand for environmental resources between dominant vegetation species in desert steppe was not very similar,hence the competition was not strong. In the process of ecological restoration of desert grassland,vegetation with wide niche width should be selected.