Effects of Folic Acid、Vitamin B12、B Vitamin Complex and Their Combination with Lysine and Methionine on Feeding Behavior and Blood Cell Parameter of Dairy Cows
[Objective]The experiment was conducted to investigate the effects of folic acid,vitamin B12(cobalamin),B vitamin complex,and their combination with lysine and methionine on feeding behavior and blood cell parameter of dairy cows.[Methods]Fifty Holstein dairy cows with similar daily milk production(44.1±5.9)kg/d and lactation days(60±38)d were randomly divided into five groups:Group Ⅰ(blank control group),Group Ⅱ(folic acid 100 mg/cow/day),Group Ⅲ(vitamin B12 175 mg/cow/day),Group Ⅳ[(thiamine 150 mg+riboflavin 150 mg+niacin 3 000 mg+pantothenic acid 475 mg+pyridoxine 120 mg+biotin 20 mg+folic acid 100 mg+cyanocobalamin 175 mg)/cow/day]and Group Ⅴ[(thiamine 150 mg+riboflavin 150 mg+niacin 3 000 mg+pantothenic acid 475 mg+pyridoxine 120 mg+biotin 20 mg+folic acid 100 mg+cyanocobalamin 175 mg+rumen escape lysine 20 g+rumen escape methionine 10 g)/cow/day].The T-test results showed no significant difference in milk production among the groups(P>0.05).[Results]The results showed that:compared with the control group,there were no abnormalities in drinking,ruminating,walking,and resting behaviors among the groups,but the feed intake of dairy cows in Group Ⅴ increased obviously;there were no significant differences in the number of white blood cells and monocytes in Groups Ⅱ,Ⅲ,Ⅳ,and V,but the numerical values had enhanced(P>0.05);there were no significant differences in the number of lymphocytes,neutrophils and platelets in Groups Ⅱ,Ⅳ and Ⅴ,but the numerical values had also enhanced,specifically,lymphocytes increased by 14.63％,12.86％and 16.72％respectively,neutrophils increased by 19.85％,16.85％and 10.67％respectively,and platelets increased by 2.96％,8.11％and 5.21％respectively(P>0.05);there were no significant differences in the number of red blood cells in Groups Ⅱ,but the numerical value increased by 0.15％(P>0.05);the hemoglobin concentration in Group Ⅱ was significantly higher than that in Group Ⅲ,with an increase of 21.74％(P<0.05),while there were no significant differences among the other groups(P>0.05).[Conclusion]In conclusion,under the conditions of this experiment,the dairy cows in treatment groups fed with folic acid,vitamin B12,B vitamin complex,and their combination with rumen escape lysine and rumen escape methionine,showed no abnormalities on feeding behavior,and the various treatments all had positive effects on blood cell parameters of dairy cows to some extent.
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