Cloning and Functional analysis of Photosynthetic-key Genes of MnPsba,MnRbcL and MnRCA In Mulberry
Leaf photosynthesis takes places in the chloroplastorgans,among which the PsbA,RbcL and RCA genes usually play crucial roles.By biological information analysis,three mulberry genes of MnPsbA,MnRbcL and MnRCA were identified with open reading frame length of 1 062 bp,1 428 bp and 1 314 bp,respectively,which encoded 353,475 and 437 amino acids in length.They differed on tertiary structures including different folding degree and spatial steering.The MnPsbA contained Photo_RC conserved domain,MnRbcL contained RuBisCO_large N domain while MnRCA contained AAA domain,respectively.These three genes were highly expressed in E1 mulberry variety,but lowly in H32 mulberry variety.Compared with the wild type,the peak value of Pn,ΦPSⅡ,qP,and the content of Chla,Chlb and total Chl in transgenic Arabidopsis were significantly upregulated.Simultaneously,the RuBP enzyme activity was verified with slight increase either.All above results showed that these three genes might play roles in mulberry photosynthesis,and through which promote to improve the yield of mulberry leaves.
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万方数据
