Effect of porous gelatin microspheres loaded with FGF10 on nerve repair in rats with spinal cord injuries:mechanism study
Objective To explore the protective mechanism of porous gelatin microspheres (GMSs) loaded with fibroblast growth factor 10 (FGF-10) in rats with spinal cord injuries (SCI). Methods Twenty healthy male SD rats (220-250 g) were randomly divided into sham operation group,SCI group,FGF10 group,and FGF10-GMSs group,with 5 rats in each group. For rats in the sham operation group,spinous process and vertebrae at T9-T10 level were removed to conduct simple bony injury without SCIs,while for rats in the other 3 groups,SCIs were conducted at the same level. After injury,20 μL FGF10 and porous GMS loaded with 20 μL FGF10 were respectively injected in-to the rat injury sites by a Hamilton microinjector in FGF10 group and FGF10-GMSs group;rats in the SCI and sham operation groups were injected with equal amount of normal saline. T9-T10 spinal cord samples were collected.An in vitro release test was conducted to assess the sustained release of FGF10 in porous GMSs. The Basso,Beattie & Bresnahan (BBB) locomotor rating scale and the slope test were used to evaluate the motor function of the experi-mental rats. Additionally,HE staining,Nissl staining,TUNEL assay,and Western blot were employed to evaluate the therapeutic effect of FGF10-GMSs on nerve recovery. Results The in vitro release test showed that regular GMSs rapidly released FGF10,while porous GMSs achieved a sustained and slow release of FGF10,without significant burst release within the initial 48 h. The BBB locomotor rating scale score showed that on the 21st day,both FGF10-GMSs group (score of 12) and FGF10 group (score of 9) demonstrated significant motor functional recovery,com-pared to the SCI group (score of 5,both P<0.05). The slope test results were consistent with BBB motor scores,in-dicating noticeable behavioral changes in both FGF10 and FGF10-GMSs groups. HE and Nissl staining revealed fe-wer necrotic,pyknotic,and infiltrating multinucleated leukocytes in the FGF10-GMSs group,resulting in a signifi-cantly reduced proportion of necrotic tissue within the spinal cord cavity. Western blot analysis indicated more pro-nounced inhibition of cell apoptosis in the FGF10-GMSs group compared to the FGF10 group. TUNEL assay also demonstrated similar results,with FGF10-GMSs showing stronger anti-apoptotic activity than FGF10 and SCI groups. Conclusion FGF10-GMSs protect neurons of SCI rats by inhibiting neuronal apoptosis,which provides a novel idea for SCI treatment.
万方数据
维普
