Serum metabolomics characteristics of pancreatic trauma in rats
Objective To explore the changes in serum metabolic characteristics after pancreatic trauma(PT)in rats and to provide new theoretical basis for studies on the mechanism and therapeutic targets of PT.Meth-ods A total of 12 SD rats were randomly divided into sham and PT groups(n=6 for each).After laparotomy,for the sham group,the pancreas of rats was gently flipped and then the abdomen was closed;while for the PT group,a modeling instrument was used to impact the pancreatic tissue at the pressure of 400 kPa.After fasting for 24 h,ser-um and pancreatic tissue sampling was conducted and HE staining was used to observe the damage to pancreatic tis-sues.The expression levels of amylase,lipase,and inflammatory factors were detected by ELISA.Liquid chromatog-raphy-tandem mass spectrometry was adopted to determine the expression of endogenous metabolites in serum,multi-variate statistical analysis to screen potential differential metabolites,and MetaboAnalyst 5.0 to analyze metabolic pathways.Results In the PT group,pancreatic acinar cells underwent degeneration and necrosis,with interstitial e-dema and enlarged gaps.The necrotic area and interstitium were accompanied by significant inflammatory cell infil-tration.Compared with the sham group,the PT group showed much higher pathological scores in terms of edema(2.33±0.19 vs.0.24±0.07),bleeding(2.28±0.16 vs.0.16±0.06),necrosis(2.52±0.09 vs.0.14±0.07),and inflammatory infiltration(2.31±0.13 vs.0.27±0.09,all P＜0.05),indicating successful PT modeling.The PT group showed much higher expression levels(pg/mL)of amylase(303.70±23.72 vs.204.70±17.67)and lipase(1207.00±89.52 vs.568.10±120.6,both P＜0.05),much higher levels(pg/mL)of IL-6(63.00±4.63 vs.12.79±1.36),TNF-α(446.50±27.96 vs.88.39±13.32)and much lower levels(pg/mL)of IL-10(22.80±3.37 vs.63.69±0.80)and TGF-β(216.90±28.14 vs.1030.00±80.70,all P＜0.05).Altogether 359 differential me-tabolites were identified in serum,of which 45 were upregulated and 314 were downregulated.These differential me-tabolites were closely related to phenylalanine metabolism,tryptophan metabolism,histidine metabolism,tricarboxylic acid cycle,and tyrosine metabolism,as well as phenylalanine,tyrosine,and tryptophan biosynthesis.Conclusion After PT in rats,there are significant changes in serum metabolomics.Energy metabolism and amino acid metabo-lism disorders are the most typical metabolic characteristics of PT,which may be related to the development of PT.Targeted callback of the relative abundance of amino acid metabolism may have certain therapeutic effects on PT.
万方数据
维普
