Multivariate mesoporous MOFs with regulatable hydrophilic/hydrophobic surfaces as a versatile platform for enzyme immobilization
Zeolitic imidazole frameworks materials-8(ZIF-8),a member of the metal-organic framework(MOFs)series,have been extensively used as a host matrix for enzyme immobilization.However,the microporous structure and hydrophobicity,as well as the protonation of the precursor 2-methylimidazole(2-MeIm)of ZIF-8,remain considerable challenges in maintaining the activity of immobilized enzymes.Here,novel multivariate mesoporous MOFs(mMOFs)with regulatable hy-drophilic/hydrophobic surfaces were designed by the multivariate competitive strategy and pore modification engineering.3-Methyl-1H-1,2,4-triazole(3-MTZ)and 5-methyltetrazole(5-MTA)were employed to partially replace 2-MeIm.These were then combined with zinc sulfate heptahydrate(soft templates)to yield mMOFs in a methanol solution.As a proof-of-concept application,we used mMOFs as carriers for enzyme immobilization and investigated the properties of the immobilized enzymes.Benefiting from their mesoporous structure,hydrophilic surface,and improved microen-vironment,multivariate mMOFs exhibit a strong ability to stabilize enzyme conformation and in-crease enzyme activity compared with traditional ZIF-8.Our study offers an avenue for the control-lable preparation of well-designed MOF structures,which will further broaden the application op-portunities of MOF materials for enzyme immobilization.
NETL
NSTL
万方数据
