A Rapid QuEChERS-GC-MS Assay for Linalool and Alpha-ionone Determinations in Tea
[Objective]An assay to detect the aromatic linalool and alpha-ionone in tea applying gas chromatography-mass spectrometry(GC-MS)was developed.[Method]Tea samples were firstly extracted by acetonitrile to collect the supernatant for the QuEChERS purification on a column containing 200 mg of PSA,200 mg of CB,and 400 mg of anhydrous sodium sulfate prior to GC-MS analysis.Under the ion monitoring mode(SIM),aromatic compounds were quantifiably determined with external standards.[Result]The assay detection limit on linalool was 0.020 mg·kg-1 and that on alpha-ionone 0.004 mg·kg-1;while the quantification limit was 0.080 mg·kg-1 on linalool and 0.010 mg·kg-1 on alpha-ionone.The measurement curves on the two aromatherapy substances were linear in concentrations of 5.0-2000.0 μg·L-1 with a greater than 0.999 correlation coefficient(R2).On the blank control sample of tea spiked with 5.0,10.0,and 1000.0 μg·L-1 of standard linalool and alpha-ionone,the assay showed an average recovery of 88.23%-106.11%with a relative standard deviation(RSD)of 1.98%-3.25%(n=6).[Conclusion]The newly developed method was rapid with a running time of only 16.8 m and highly reproducible,accurate,and sensitive results.It was deemed applicable for rapid detection of linalool and alpha-ionone in tea.
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