To study the in vitro inhibitory effects of tea phenolic components and their combinations on P.aeruginosa PAO1,and the best antibacterial prescription with the lowest dosage was screened.The minimum inhibitory concentration(MIC)of five phenolic components on P.aeruginosa PAO1 was determined by multiple ratio dilution method combined with the color rendering of resazurin,and the response surface method was used to study the optimal compatibility with strong inhibitory effect on P.aeruginosa PAO1.The results showed that all five phenolic components had different degrees of inhibitory effects on P.aeruginosa PAO1.The antibacterial activity of the five tea active components on P.aeruginosa PAO1 was EGCG>TF60=ECG>EGC>EC.The results of response surface analysis showed that EGCG,EGC,ECG and TF60 had the best bacteriostatic effect on P.aeruginosa PAO1 when they were combined in the ratio of 1.0:4:4.81:4.0.Conclusion:The combination of EGCG,EGC,ECG and TF60 has obvious inhibitory effect on P.aeruginosa PAO1.
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