11种凤凰单丛茶中9种黄酮酚类物质的检测方法建立与分析
Establishment and Analysis of Detection Methods for 9 Flavonoid Phenols in 11 Types of Fenghuang Dancong Tea
陈惜燕 1邵跃桁2
作者信息
- 1. 西北农林科技大学生命科学学院,陕西杨凌 712100
- 2. 汕头市归一堂文化传播教育中心,广东汕头 515824
- 折叠
摘要
建立了高效液相色谱法(HPLC)同时测定凤凰单丛茶中9种黄酮酚类物质的检测方法.采用色谱柱Waters Xterra®MS C18(4.6 mm×250 mm,5μm),以乙腈和0.02％乙酸为流动相进行梯度洗脱,流速1.0 mL/min,柱温25℃,二极管阵列检测器280 nm波长检测,外标法定量.结果表明,9种黄酮酚类物质中香豆酸、绿原酸、儿茶素、芦丁和槲皮素在0.01～0.20 mg/mL,木犀草素、金丝桃苷、山奈酚和槲皮苷在0.025～0.250 mg/mL线性关系良好,相关系数(r)均大于0.999;方法检出限为0.2～1.8μg/g,定量下限为0.6～5.9μg/g.在25μg/g和250μg/g两个加标水平下,9种黄酮酚类物质的平均回收率为95.4％～104.8％,相对标准偏差RSD(n=6)均小于5％.该方法操作简便、快速、准确,适合于黄酮酚类物质的定性与定量检测.
Abstract
A detection method for the simultaneous determination of nine flavonoid phenols in Fenghuang Dancong tea by high performance liquid chromatography (HPLC) was established. Column Waters Xterra® MS C18 (4.6 mm × 250 mm,5 μm),acetonitrile and 0.02％ acetic acid were used as mobile phases for gradient elution with flow rate of 1.0 mL/min,column temperature of 25℃,diode array detector 280nm wavelength for detection,external standard method for quantification. The results showed that among the nine flavonoid phenols,coumaric acid,chlorogenic acid,catechin,rutin and quercetin had a good linear relationship at 0.01～0.20 mg/mL,and luteolin,hypericin,kaempferol and quercetin had a good linear relationship at 0.025～0.25 mg/mL,and the correlation coefficient (r) was greater than 0.999;The detection limit of the method was 0.2～1.8 μg/g,and the lower limit of quantification was 0.6～5.9μg/g. At two spiking levels of 25 μg/g and 250 μg/g,the average recovery rate of 9 flavonoid phenols was 95.4％～104.8％,and the relative standard deviation RSD (n=6) was less than 5％. This method is simple,fast and accurate,and is suitable for the qualitative and quantitative detection of flavonoid phenols.
关键词
凤凰单丛茶/黄酮酚类物质/高效液相色谱法(HPLC)Key words
Fenghuang Dancong tea/Flavonoid phenols/High Performance Liquid Chromatography(HPLC)引用本文复制引用
基金项目
西北农林科技大学实验技术研究与实验室管理创新项目(SY20210211)
出版年
2024
国家科技期刊平台
NSTL
万方数据