Safety Evaluation of Nicotinic Insecticide Dinotefuran on Honeybee ( Apis mellifera L. )
As a novel neonicotinoid pesticide, what effect dinotefuran plays on bee remains unclear. The aim of this study was to determine both acute and chronic oral toxicity, larvae toxicity of dinotefuran to Italian honeybees (Apis mellifera L.). Risk assessment of this pesticide was also performed based on hazard quotients (HQ). Results showed that the LD50 values obtained after 48 h (acute oral toxicity) and 10 d (chronic oral toxicity) of dinotefuran exposure were 0.033 μg·bee-1 and 0.010 μg·bee-1, respectively. The chronic toxic effect zone was 3.5, suggesting a risk of chronic poisoning. The LD50 value against larvae obtained after 7 d dinotefuran exposure was 577 ng·lar-vae-1 . Moreover, the survival rate, pupation rate and emergence rate were negatively correlated to the doses of di-notefuran (r= -0.98, -0.89 and -0.80 respectively). According to the HQ value, dinotefuran has medium to high risk to honeybees. The results indicated that the application of dinotefuran to control pest in field should be careful-ly considered since it may have potential environmental safety problems.