Effects of Colistin Sulphate on nirS, nosZ Gene Diversity of Soil Denitrification Bacteria
Based on the colistin sulphate exposure experiments, the nirS, nosZ gene diversity of soil denitrification bacteria exposed were analyzed by using the terminal restriction fragment length polymorphism (T-RFLP) methods.The total Operational Taxonomic Units (OTU) of nirS and nosZ gene is lower than that of the control group. Furthermore, the changes of nirS gene diversity were dependent on the dose of colistin sulphate. The nirS genes of dominant bacteria mainly concentrated in 10 segments while nosZ genes in 18 segments in each group. The richness of nir S gene has lager changes than that of nosZ gene. There exist significant differences for the Shannon index of nirS genes between high concentration groups and the control groups on the 7 thday of incubation (P＜0.05). On the7 thday, 49 thday, there exist significant differences for Pielou index between the high concentration groups and the control group (P＜0.05). On the 35 thday, there are significant differences for the Shannon index of nir S gene between the intermediate concentration groups and the control groups (P＜0.05). The Simpson index on 21 stday, 35 th day for the low concentration groups is significantly different from those of the control group (P＜0.05). However, the differences in Pielou index are not significant among each group (P＞0.05). When the drug concentration ≥5 mg·kg-1, the evenness and diversity of the soil denitrification bacteria decreased and the community dominance increased simultaneously, which affected the nirS gene significantly. Our results suggested nirS genes were suitable as a reporter gene for colistin sulfate pollution.
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NSTL
万方数据
维普
