Chronic Toxic Effects of Kresoxim-methyl during Zebrafish Early Life Stage
In order to investigate the chronic toxicity of strobilurins to the early life stage of aquatic organisms,the sublethal effect of kresoxim-methyl on the early development of zebrafish after long-term exposure was determined.After continuous exposure of zebrafish embryos to different concentrations of kresoxim-methyl(0.000750～0.180 mg·L-1)for 34 d,the median lethal concentrations(LC50)of kresoxim-methyl for 144 h and 34 d of zebrafish early life stage was 0.141 mg·L-1 and 0.0106 mg·L-1,respectively.It was found that 0.00460 mg·L-1,0.0115 mg ·L-1 and 0.0288 mg·L-1 kresoxim-methyl significantly decreased the body weight of larvae after 34 d exposure,0.0115 mg·L-1 and 0.0288 mg L-1 kresoxim-methyl decreased the body length of larvae,and 0.180 mg·L-1 kresoxim-methyl increased the body length and body weight of larvae.The 34 d-NOEC and 34 d-LOEC of kresoxim-methyl was 0.00460 mg·L-1 and 0.0115 mg·L-1 for body length,and was 0.00185 mg·L-1 and 0.00460 mg·L-1 for body weight during zebrafish early life development,respectively.During the 34 d exposure,the activi-ties and levels of factors related to mitochondrial energy metabolism and oxidative stress in zebrafish embryos/larvae were measured at 1,3,7,14,21 and 34 d.Results showed that long-term exposure of kresoxim-methyl could increase the catalase(CAT)activity,the peroxide(POD)activity increased then decreased and then increased,the superoxide dismutase(SOD)activity and malondialdehyde(MDA)content decreased first then increased during zebrafish early life development.It was found that the calcium ion(Ca2+)level and the activity of calcium ion adenosine triphosphatase(Ca2+-ATPase)was inhibited during the 34 d exposure of kresoxim-methyl,and the adenosine triphosphate(ATP)level was increased first then decreased.The results suggested long-term exposure to kresoxim-methyl caused negative effects on the growth indexes and antioxidant system during zebrafish early life development,kresoxim-methyl might interfere with mitochondrial energy metabolism by regulating the ATP and Ca2+levels,and Ca2+-ATPase activity,then induced oxidative stress during the early life stage of zebrafish.
kresoxim-methylzebrafishchronic toxic effectearly life stageoxidative stress
万方数据
维普
