Biotoxic Effect of Oil-based Drilling Cuttings on Maize in Shale Gas Development in Southern Sichuan
Taking the oil-based drilling cuttings derived from the process of shale gas development in southern Si-chuan as the research object,the influence of adversity stress of different mass percentages of oil-based cuttings on the growth targets during the growth process of maize were investigated.The growth targets included biomass indexes(such as seed emergence rate,height over land,stem and leaf fresh weight,stem and leaf dry weight,root length,root fresh weight and root dry weight)and physiological indexes of leaf chlorophyll,hydrogen peroxide(H2O2)and malondialdehyde(MDA)contents.When the mass percentage of oil-based drilling cuttings was 100％,the germination rate of maize was still 86.67％,reflecting the slight influence of oil-based drilling cuttings.The growth of maize seedling under the stress of different mass percentages of oil-based drilling cuttings presented the phenomenon of low concentration promotion and high concentration inhibition.Generally,the differences between various growth indexes were slightly significant(P＜0.05).Among them,the plant height and fresh weight of roots in the later period of maize cultivation(30 d)were significantly affected(P＜0.001)when the mass percentage of oil-based drilling cuttings was 100％.The oil-based drilling cuttings had significant effect on the mass concentra-tion of chlorophyll and the molar concentrations of H2O2 and MDA in maize leaves(P＜0.01)if its mass percentage was ≥ 50％.With the prolonged planting time and the increasing mass percentage of oil-based drilling cuttings,the mass concentration of chlorophyll in maize leaves decreased,whereas the molar concentrations of H2O2 and MDA in leaves increased.When the mass percentage of oil-based drilling cuttings was 100％,the mass concentration of chlorophyll in the withered yellow leaves was only 0.15 mg·g-1.At the same time,the molar concentrations of H2O2 and MDA were 0.09 nmol·g-1and 25.91 μmol·g-1,respectively,which were 7.5 and 3.5 times of the blank control group.Therefore,the bio-toxic effects of oil-based drilling cuttings on maize could be demonstrated as the"low concentration promotion and high concentration inhibition".The doping of oil-based drilling cuttings with a low mass percentage concentration(≤25％)had little effect on the growth of maize,which offers the possibility of using biodegradation and crops to repair the oil-based drilling cuttings derived from the process of shale gas development.Meanwhile,it also provides a certain theoretical support for realizing the crop reclamation on the platform of shale gas development.
cadmiumoil-based drilling cuttingsmaizephytotoxicityshale gas development
万方数据
维普
