Assessment of Diversity of Local Terrestrial Plant Communities by Environmental DNA Technology
Environmental DNA(eDNA)has emerged as a crucial tool for assessing biodiversity across various dis-ciplines and regions.However,there has been limited research on using airborne eDNA for terrestrial plant diversi-ty monitoring in tropical areas.To assess the feasibility of utilizing airborne eDNA to detect plant species diversity in tropical regions,we deployed eDNA collectors at Hainan University(Haidian Campus),with wind speeds ran-ging from 0～3.5 m·s-1.We collected eDNA samples at seven different time points and employed an improved DNA extraction method,with Rbcl chosen as the amplification primer for our study.Results indicated that using homemade airborne eDNA devices and an enhanced DNA extraction method enabled the capture of eDNA meeting sequencing quality requirements.Sequencing analysis of collected eDNA revealed a total of 2 262 operational taxo-nomic units(OTUs),annotated to 841 plant species from 543 genera across 44 families,far surpassing existing campus plant records.Detection rates of spore-bearing plants and wind-pollinated flowering plants using eDNA technology were approximately 55.5％,not significantly higher than other plant categories.The shared OTUs among the seven treatments totaled 171,with the highest OTU count and biodiversity index observed on the 10th day.Our study suggests the feasibility of using airborne eDNA technology for studying and monitoring terrestrial plant species in tropical regions.
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