Effects of High Altitude Hypoxic Environment on Reproductive System of Female Rats
To investigate the effects of exposure to plateau hypoxia on the reproductive system of female rats.Twenty female Wistar rats were physiologically synchronized and randomly divided into Plain group and Plateau group.The Plain group was placed in Lanzhou(1 500 m above sea level),while the Plateau group was transported to Batang,Yushu,Qinghai Province(4 010 m above sea level)and then exposed to plateau hypoxia for 14 days.During the experiment,the body weight and estrous cycle changes were recorded,the organ index was calculated from each tissue and organ.The changes in reproductive hormone levels and oxidative stress levels in serum,ovary and uterus tissues were detected,HE staining was used to observe the morphology of ovary and uterus tissues,and the expression of hormone receptor proteins in ovary tissues was detected by protein blotting method.Compared with the Plain group,rats in the Plateau group showed significant weight loss,estrous cycle disruption,up-regula-tion of oxidative stress and reproductive hormone levels in serum,ovary and uterus tissues,and HE staining showed that the morphology and structure of ovary and uterus in the Plateau group were intact,whereas the structure of ovary and uterus in the Plateau group was damaged.The amount of hormone receptor proteins in the ovarian tissue was reduced compared with that in the Plateau group.Hypoxia in the Plateau group caused reproduc-tive damage in female rats.
high altitude hypoxiafemale Wistar ratsoxidative stresshormoneestrous cycle
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