Acute Toxicity and Toxicology of Tetraniliprole on Daphnia magna
Tetraniliprole is a new generation of diamide insecticide,which has broad application prospects,and it is important to clarify its environmental safety.In this study,the toxic effects of tetraniliprole on the aquatic organism Daphnia magna were evaluated.The present results show that tetraniliprole has a high risk of acute toxicity to Daphnia magna,evidenced by a LC50 value of(0.096±0.010)mg·L-1 at 48 h.At concentrations of 0.005 mg·L-1 and 0.025 mg·L-1,tetraniliprole inhibited the feeding behavior and motor behavior of Daphnia magna to varying degrees.Additionally,the activities of superoxide dismutase(SOD)in Daphnia magna were significantly enhanced by tetraniliprole,while the activities of acetylcholinesterase(AChE),glutathione S-transferase(GST)and trypsin were significantly inhibited.Transcriptome sequencing results showed that the number of differentially expressed genes(DEGs)induced by tetraniliprole increased gradually with rising exposure concentrations.The KEGG enrich-ment analysis showed that the metabolism and protein synthesis related signaling pathways were significantly affected by different doses of tetraniliprole.Screening and verification of key DEGs showed that the expression levels of antioxidant-related genes were significantly up-regulated in each tetraniliprole exposure treatment,while the expression levels of genes associated with detoxification,ribosome function and digestion were significantly down-regulated in each tetraniliprole exposure treatment.This study not only elucidates the acute risk of tetraniliprole to Daphnia magna but also unveils the physiological effects of tetraniliprole on the function of Daphnia magna at sublethal concentrations.The obtained insights can provide theoretical basis for the environmental safety of tetranil-iprole in aquatic environment.
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