Impact of Co-exposure to Methylmercuric Chloride and Selenomethionine on Mercury Accumulation and Toxicity in Rats
The aim of this study is to provide important support for further research on the accumulation and trans-port mechanisms of mercury and selenium in vivo by exposing rats to methylmercury chloride and selenomethionine.Cold atomic fluorescence spectroscopy(CVAFS)was used to detect the mercury content in rats;hydride occurrence by atomic fluorescence spectroscopy(HG-AFS)was used to detect the selenium content in rats;hematoxylin-eosin(HE)staining was used to observe tissue morphology;One-way ANOVA and Bonferroni methods were used to analyze the differences in mercury and selenium distribution in rats,and Independent sample T-test was used to analyze the differences in liver and kidney function between groups.The results showed that the accumulation of mercury and selenium in male mice was higher than that in female mice of different genders,and the accumulation of mercury and selenium in rats of the same gender was in the order of kidney＞liver＞brain＞hair ≈ feces,and the accumulation of mercury gradually increased with time.The accumulation of selenium in the liver,kidney,and brain of rats exposed to selenium continued to increase with the extension of exposure time from 30 to 60 d,and a phenomenon of decline appeared at 90 d;in the combined selenium and mercury combined exposure group,under the same mercury exposure conditions,the mercury accumulation in each tissue also increased with the increase of selenium exposure dose;after selenium supplementation,both mercury and selenium accumulation increased,but the mercury accumulation was lower than that of the single mercury group;from biochemical indicators and pathological observations,compared with the control group,the single mercury group and the medium selenium high mercury group had more severe tissue damage in rats,while the single selenium group had the lowest degree of damage.In summary,the interaction between selenium and mercury can affect the accumulation and distribution of mercury and selenium in tissues.Co exposure to selenium or supplementation with selenium can promote the absorption of mercury in various tissues,and the results are influenced by the exposure level and supplementation concentration of selenium and mercury.The accumulation of mercury and selenium can affect the degree of pathological changes in various tissues and liver and kidney function in rats.The higher the accumulation of mercury,the greater the degree of tissue damage.
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