Effects of CuSO4 on Cyanobacterium(Microcystis aeruginosa)in Presence of Nanoplastics
In this study,we explored the effects of CuSO4 on a toxigenic strain of Cyanobacterium(Microcystis aeruginosa)in the presence of polystyrene nanoplastics(PS NPs).We investigated the individual impact of three different functionalized PS NPs(PS-COOH,PS,PS-NH2),CuSO4,and their combined effects on cell density,chlo-rophyll a(Chi a),malondialdehyde(MDA)content and superoxide dismutase(SOD)activity.The results demon-strated that the combination of PS NPs alleviated the toxicity of CuSO4 to Cyanobacterium.Compared with the blank control(CK),after exposure to CuSO4,PS-COOH+CuSO4,PS+CuSO4 and PS-NH2+CuSO4,the cell density of Cyanobacterium was inhibited by 42％,7％,5％and 36％,respectively,and the Chi a content was decreased by 55％and 6％,7％and 45％.The results indicated that the co-exposure of PS-NH2 and CuSO4 had more signifi-cant inhibitory effects on Cyanobacterium growth and chlorophyll synthesis than the other two PS NPs.According-ly,PS-COOH and PS significantly alleviated the toxic stress of CuSO4 on the activities of MDA and SOD in Cya-nobacterium.Compared with blank control,the MDA content and SOD activity in Cyanobacterium exposed to PS-COOH+CuSO4 and PS+CuSO4 treatment groups were increased by 31％and 35％,as well as 7％and 5％,respec-tively.While MDA content and SOD activity in Cyanobacterium exposed in CuSO4 and PS-NH2+CuSO4 treatment groups were increased by 99％and 66％,as well as 22％and 5％,respectively.Similarly,except for PS-NH2,the other two PS NPs can significantly reduce the extracellular microcystins(MCs)release from Microcystis aeruginosa during CuSO4 treatment.Our findings illustrate the importance of taking the nanoplastics into account before CuSO4 is applied to control cyanobacteria bloom.
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