Sublethal Effects of Pyraclostrobin on Zebrafish during Early Life Stages
To assess the sublethal effects and chronic toxicity of pyraclostrobin on zebrafish early life stage,the impact on the growth,oxidative stress,energy metabolism and enzymes involved in liver energy metabolism were determined after zebrafish embryos exposed to various concentrations(0.328～32.0 μg·L-1)of pyraclostrobin for 30 d.Result showed 5.12 μg·L-1 pyraclostrobin reduced the body length of larvae,2.05,5.12,and 12.8 μg·L-1 pyraclostrobin increased the body weight after 30 d exposure,while 0.819,5.12 and 12.8 μg·L-1 pyraclostrobin caused different degrees of vacuolation in larvae liver.To further clarify the toxic effects of pyraclostrobin during zebrafish embryonic development stages,the contents of pyruvate,glucose,cholesterol,triglycerides and free fatty acids,and the enzymes related to oxidative stress and liver energy metabolism were determined at 2 d,6 d,10 d,16 d,and 30 d.During the exposure,it was found the activities of catalase(CAT)first decreased and then increased,exposure to pyraclostrobin also affected the peroxide dismutase(SOD)activity and free fatty acids level during zebrafish different early life stages,the content of pyruvate decreased first then increased,the triglyceride levels decreased overall during 30 d exposure,while pyraclostrobin induced glucose and total cholesterol levels during zebrafish early different life stages,significantly increased the glucose levels at 6 d and 30 d.In addition,pyraclostrobin induced the alkaline phosphatase(AKP)activity during the early life stages of zebrafish,also affected the alanine aminotransferase(GPT)and glutamic-oxaloacetic transaminase(GOT)activities during 30 d exposure.The results suggested pyraclostrobin might induce oxidative stress,interfered with the synthesis of glucose and total cholesterol,affected the antioxidant system and energy metabolism in zebrafish,then altered the enzyme activity involved in liver energy metabolism further disturbed the energy metabolism,which caused negative effects on the growth and liver development during the early life stages of zebrafish.
pyraclostrobinzebrafishchronic toxicityearly life stage
NETL
NSTL
万方数据
