首页|菜子湖线有机磷酸酯污染及其与微生物群落特征相关性的初步分析

菜子湖线有机磷酸酯污染及其与微生物群落特征相关性的初步分析

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有机磷酸酯(organophosphate esters,OPEs)的广泛应用导致其在环境累积并凸显其日益严峻的生态毒性问题.研究有机磷酸酯对引江济淮工程菜子湖流域沉积物微生物群落及其功能基因的影响,有助于我们认识这类新型污染物的生态风险并制定有效防控措施.本研究通过实地采样分析了2021年11月至2022年11月间菜子湖线流域水体中OPEs的时空分布特征,并运用16S rRNA基因高通量测序技术对沉积物微生物进行分析,结合主坐标分析(PCoA)和冗余分析(RDA),探究了OPEs浓度变化与微生物群落结构的关系,同时借助PICRUSt预测了微生物功能基因.研究表明,菜子湖线水域∑OPEs浓度均值从2021年11月的167.55 ng·L-1上升至2022年11月的177.48 ng·L-1.氯代类OPEs如磷酸三氯乙酯(TCEP)和磷酸三氯丙酯(TCPP)为主要污染成分,浓度分别达到数百ng·L-1级别.在微生物群落方面,变形菌门、厚壁菌门和拟杆菌门在较高OPEs浓度下相对丰度显著增加,其中相对应的菌属与OPEs含量呈正相关.通过FAPROTAX预测功能分析,结果显示微生物群落具有化学异养、需氧化学异养、芳香化合物降解及硫化合物暗氧化等功能活性,暗示了该水域微生物可能具备对OPEs及其他有机污染物的降解能力.同时,结合PICRUSt预测数据,微生物组功能主要涉及氨基酸代谢、碳水化合物代谢等关键通路,进一步表明微生物可能通过这些途径参与OPEs的生物转化过程.本研究表明,菜子湖线水域沉积物微生物群落结构受到OPEs污染影响,特定菌门的丰度变化可能反映出其对OPEs的潜在降解能力,为进一步探讨OPEs的环境行为及微生物修复机制提供了科学依据.
Preliminary Analysis of Organophosphate Contamination in Caizi Lake Line and Its Correlation with Microbial Community Characteristics
The widespread application of organophosphate esters(OPEs)in various products has escalated concerns over their environmental accumulation and ecological toxicity.Studies on the impact of OPEs on sediment microbial community structure and functional genes in the Caizi Lake Line within the Yangtze River Diversion Project can facilitate our understanding of the ecological risks posed by such novel organic pollutants and help to formulate effective control strategies.Field sampling was conducted from November 2021 to November 2022 and the temporal-spatial distribution characteristics of OPEs in the water bodies was analyzed.The sediment microbial was analyzed by employing high-throughput sequencing technology targeting 16S rRNA.The relationship between OPEs concentrations and the microbial community structure was investigated using Principal Coordinate Analysis(PCoA)and Redundancy Analysis(RDA),and PICRUSt was used to predict the functional genes of these microbes.The findings indicate that the average concentration of ∑OPEs in the Caizi Lake Line increased from 167.55 ng·L-1 in November 2021 to 177.48 ng·L-1 in November 2022.Chlorinated OPEs such as tri(2-chloroethyl)phosphate(TCEP)and trichloropropyl phosphate(TCPP)were the primary contaminants,the concentrations of which both reached hundreds of ng·L-1.As for the microbial community,the relative abundance of Proteobacteria,Firmicutes,and Bacteroidetes significantly increased at higher OPEs concentrations,and the corresponding genera of those phyla were positively correlated with the OPEs content.The FAPROTAX prediction analysis showed that the microbial community exhibited functional activities of chemoheterotrophy,aerobic chemoheterotrophy,aromatic compound degradation,and dark sulfur compound oxidation,suggesting the potential for degrading OPEs and other organic pollutants.In addition,PICRUSt predictions indicated that the functions of microbial community mainly involved key pathways of amino acid and carbohydrate metabolism,suggesting that microbes participate OPEs biotransformation through these pathways.In conclusion,this study demonstrates that the sediment microbial community structure in the Caizi Lake Line is affected by OPE pollution,and the changes of the abundance of some phyla reflecting their potential degradation capacity for OPEs.These findings provide a scientific basis for further investigation into the environmental behavior of OPEs and the underlying mechanisms of microbial remediation.

OPEsmicrobial communitydiverting water from the Yangtze River to the Huai River16S rRNA high-throughput sequencingfunctional prediction

黄风云、马昆、谷鹏飞、王李耘、谈凯、宋婧、赵凯

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安庆师范大学生命科学学院,安庆 246133

安徽省流域生物多样性保护与利用"一带一路"示范型国际科技合作基地,安庆 246133

OPEs 微生物群落 引江济淮 16S rRNA高通量测序 功能预测

安徽省高等学校自然科学研究重点项目安徽省高等学校自然科学研究重点项目安徽省高等学校自然科学研究优创团队项目水生生物保护与生态修复安徽省高等学校工程技术研究中心开放基金水生生物保护与生态修复安徽省高等学校工程技术研究中心开放基金

2022AH0510362022AH0525462023AH010041Sq2019003ASSK017

2024

10.7524/AJE.1673-5897.20231231001

生态毒理学报
中国科学院生态环境研究中心

生态毒理学报

CSTPCD北大核心
影响因子：0.857
ISSN：1673-5897
年,卷(期)：2024.19(4)