Effect of Low-Level PFOA Stress on the Physiological and Biochemical Characteristics of Soybean
Perfluorooctanoic acid(PFOA)is a persistent environmental contaminant with widespread distribution and high resistance to degradation,posing significant ecological risks.This study employed pot experiments to as-sess the impacts of soil PFOA contamination at 100 ng·g-1 on key physiological and biochemical parameters in soybean(Glycine max)leaves,including soluble protein content,malondialdehyde(MDA)content,antioxidant ca-pacity,photosynthetic pigment content,and photosynthetic performance.The results revealed that PFOA exposure caused a 21.3％decrease in root dry biomass and a 14.4％reduction in shoot dry biomass.Soluble protein content in the leaves decreased by 15.5％at the second trifoliate(V2)stage,while total phenol content decreased signifi-cantly by 61.9％at the full seed(R6)stage.Moreover,DPPH radical scavenging activity in the leaves increased by 21.1％and 23.6％at the V2 and R6 stages,respectively.PFOA stress disrupted soybean growth and development by activating the plant's antioxidant defense mechanism,as indicated by a reduction in lipid peroxidation.However,as exposure persisted,the balance between the production and scavenging of free radicals became increasingly dis-rupted,leading to the accumulation of free radicals.This imbalance resulted in a decline in total phenol content,which was insufficient to counteract the elevated levels of reactive oxygen species.Despite these stress conditions,soybeans demonstrated an adaptive response by activating their antioxidant systems to mitigate the impacts of low-level PFOA exposure.
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