Screening,Identification and Degradation Characteristics of Galaxolide Degrading Bacteria
The synthetic musk galaxolide(HHCB)is a new pollutant among pharmaceuticals and personal care products(PPCPs),which accumulates widely in environmental media due to its unique persistence and lipophilicity,resulting in significant environmental pollution.In order to investigate the microbial remediation mechanism of syn-thetic musk,a strain H10 was screened from contaminated soil samples by enrichment,isolation,and domestication methods.It exhibited excellent degradation ability towards HHCB.The strain H10 was preliminarily identified as the Chryseobacterium sp.,based on morphological characteristics,physiological and biochemical analysis,as well as 16S rRNA sequence comparison.The most suitable optimal conditions for HHCB degradation were determined as follows:inoculation amount of 5％,pH 7.0,temperature of 30 ℃.After 72 h of incubation under these condi-tions,the degradation rate of HHCB at a concentration of 200 mg·L-1 reached 68.07％.The results of the response surface experiment indicated that when the inoculation amount was adjusted to 5.95％,pH to 7.4,temperature to 30.55 ℃ and substrate concentration remained at 200 mg·L-1 after optimization for bacterial density reached up to 0.662 after incubation for another period of time while achieving a degradation rate of HHCB at approximately 70.15％.The study found that the strain H10 had the ability to degrade HHCB,which not only enriches the HHCB degrading bacterial library,but also provides a theoretical basis for microbial remediation of environments contami-nated with artificially synthesized musk.
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