摘要
为探讨羧基化单壁碳纳米管(SWCNTs-COOH)的生物效应,本研究选用"品育8012"小麦为实验材料,采用水培的方式研究不同浓度SWCNTs-COOH(0、5、10、30、50、70和90 mg·L-1)对低钾(K+,0.01 mmol·L-1)胁迫下小麦幼苗生长发育的影响.结果表明:5～50mg·L-1的SWCNTs-COOH对小麦幼苗的生长指标(株高、根系、叶面积、鲜质量、干质量)、光合作用指标(细胞色素含量、叶绿素荧光参数)、抗氧化酶(SOD、POD)活性均存在一定的促进作用,其中50 mg·L-1浓度时促进效果最明显;当SWCNTs-COOH的浓度达到70 mg·L-1及以上时小麦幼苗的生长指标、光合作用指标、SOD活性、POD活性逐渐降低,丙二醛(MDA)含量明显上升,即高浓度的SWCNTs-COOH加重了低钾胁迫下小麦幼苗的毒理学效应.综上,SWCNTs-COOH对低钾胁迫下小麦幼苗生长发育的影响存在明显的浓度效应,低浓度的SWCNTs-COOH可以缓解低钾胁迫对小麦幼苗造成的部分损伤,高浓度的SWCNTs-COOH会对小麦幼苗产生一定的生理毒性.
Abstract
To explore the biological effects of carboxylated single-walled carbon nanotubes(SWCNTs-COOH),this work selected"Pinyu 8012"wheat as the experimental material and used hydroponics cultivation method to investigate the effects of various concentrations of SWCNTs-COOH(0,5,10,30,50,70,and 90 mg·L-1)on the growth and development of wheat seedlings under low potassium stress(K+,0.01 mmol·L-1).With the most nota-ble impact being observed at a concentration of 50 mg·L-1,the results revealed that SWCNTs-COOH concentra-tions ranging from 5 to 50 mg·L-1 positively affected growth parameters(plant height,root system,leaf area,fresh weight,dry weight),photosynthetic indicators(cytochrome content,chlorophyll fluorescence parameters),and an-tioxidant enzyme(SOD,POD)activities in wheat seedlings.However,at doses of 70 mg·L-1 or higher,growth in-dicators,photosynthetic parameters,SOD activity,and POD activity in wheat seedlings dropped while the malondial-dehyde(MDA)content rose dramatically.This implies that high quantities of SWCNTs-COOH exacerbate the toxi-cological effects of low potassium stress on wheat.In summary,there is a significant concentration effect of SWCNTs-COOH on the growth and development of wheat seedlings under low potassium stress.Low concentra-tions of SWCNTs-COOH can help to reduce some of the low potassium stress-induced damage in wheat seedlings,while high concentrations of SWCNTs-COOH can cause certain physiological toxicity to wheat seedlings.
维普
万方数据