The aim of this study is to investigate the effect of 0.5 pig·mL-1 graphene oxide-silver nanoparticles(GO-AgNPs)for long time treatment on the differential expression of lncRNAs in embryonic cells.Caprine fetal fi-broblasts were treated with 0 μg·mL-1 or 0.5 pig·mL-1 GO-AgNPs for 70 days,and RNA were collected for high-throughput sequencing.Differentially expressed(DE)lncRNAs were analyzed by GO and KEGG pathways.The DE lncRNAs were confirmed by quantitative real-time PCR.The results demonstrate that a total of 167 lncRNAs are significantly different in the treated group compared to control,of which 72 are up-regulated and 95 are down-regulated.GO analysis shows that DE genes are associated with single-organism cellular processes and tissue migration related to regulation of cellular component movement,etc.KEGG pathway analysis shows that the DE lncRNAs are mainly related to the biosynthesis of interleukin-2,etc.In conclusion,long-term treatment of caprine fetal fibroblast cells with non-lethal dose of GO-AgNPs can induce changes in lncRNAs expression profile.
维普
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