Immunotoxic Effect of Plasticizer BBP in Aggravating Allergic Asthma
The aim of this study is to investigate the mechanism of the plasticizer butyl benzyl phthalate(BBP)in exacerbating allergic asthma.This study included male BALB/c mice,which were randomly divided into the fol-lowing five groups,with a modeling period of 37 days:(1)physiological saline group,(2)ovalbumin(OVA)group,(3)10 mg·kg-1 BBP+OVA group,(4)100 mg·kg-1 BBP+OVA group,and(5)250 mg·kg-1 BBP+OVA group.The study primarily assessed total immunoglobulin E(T-IgE),OVA-specific immunoglobulin E(OVA-IgE),interleukin-17,and interleukin-22.Additionally,endoplasmic reticulum stress markers activating transcrip-tion factor-6(ATF6)and C/BEP homologous protein(CHOP),T regulatory(Treg)pathway molecules interleu-kin-10,and forkhead box protein P3 in immunohistochemical sections of lung tissue were analyzed.Lung func-tion was evaluated by observing lung tissue changes and using a noninvasive ventilator.The results showed that compared with the saline group,the T-IgE,OVA-IgE function,lung function,and pathological damage in the OVA group were all aggravated,while the Th17/Treg balance was disrupted.Compared with the OVA group,as the concentration of BBP increased,the lung function,T-IgE,OVA-IgE function,and pathological damage in the different concentrations of BBP+OVA groups progressively worsened,shifting the balance toward Th17,with significant differences at 100 mg·kg-1.Furthermore,the experimental results revealed that BBP exacerbates aller-gic asthma by regulating the Thl7/Treg pathway through endoplasmic reticulum stress,with a significant effect noted at a BBP dose of 100 mg·kg-1.
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维普
