Toxic Effects of Tetracycline and Metronidazole on Chlorella pyrenoidosa
In recent years,the problem of antibiotic pollution in the aquatic environment has become increasingly prominent.To assess the ecological risk level of tetracycline(TC)and metronidazole(MTZ)to green algae,Chlo-rella pyrenoidosa was used as a classical model organism to design growth inhibition experiments in this study.The growth and oxidative stress response of Chlorella pyrenoidosa were analyzed by detecting changes in algal cell density,surface functional groups,extracellular polymers,and chlorophyll a content under different concentrations of TC and MTZ stress.The results showed that after seven days of exposure to TC(15 mg·L-1)and MTZ(50 mg·L-1),the inhibition of algal density reached 84％and 85％,respectively,and the concentrations of chlorophyll a(Chl-a)were both reduced by more than 80％.FTIR analysis showed that specific peaks were shifted in both TC and MTZ test groups and the amide Ⅱ band absorption peaks completely disappeared in the TC group.In addition,the extracellular polymers were also affected by the changes in TC and MTZ concentrations to different degrees.The above experimental results showed that TC and MTZ had more significant toxic effects on Chlorella,and the toxicity of TC was higher than that of MTZ.
tetracyclinemetronidazoleChlorella pyrenoidosagrowth inhibitionchlorophyll a
万方数据
维普
