A method was developed for the ultrasonic extraction of phoxim and lambda-cyhalothrin from soil and the detection of their residues by high performance liquid chromatography.The soil samples were ultrasonic extraction by acetonitrile-water(4∶1,by vol)as extractant.The extract was purified with Floridian column.The residue was determined by reversed phase high performance liquid chromatography.With Synergi™ Polar-RP 80 Å(250 mm×4.6 mm,4 μm)column was used as the fix phase,the mixture of acetonitrile and water(80∶20,by vol)was used as the mobile phase,the UV detector wavelength was 225 nm.Results show that both phoxim and lambda-cyhalothrin exhibit extraction rates exceeding 92％.The concentration and peak area of phoxim exhibit a good linear relationship between 0.6～5.4 μg/mL,and the linear regression equation is A=25.108ρ+45.008,r=0.998 9.The concentration and peak area of high-efficiency cypermethrin exhibit a good linear relationship between 0.4～3.6 µg/mL,and the linear re-gression equation is A=48.835ρ+56.574,r=0.999 0.The method is simple,rapid,of high accuracy and precision,and can meet the requirements for detecting phoxim and lambda-cyhalothrin residues in soil.
关键词
高效液相色谱/辛硫磷/高效氯氟氰菊酯/土壤/残留
Key words
high performance liquid chromatography/phoxim/lambda-cyhalothrin/soil/residue
维普
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