Determination of Anthocyanin Conten in Crataegi Fructus by pH-differential method
Objective:To develop a quantitative method for determining the contents of anthocyanin in crataegi fructus. Methods:Crataegi fructus was extracted with 30% ethanol,the aqueous extract was diluted with pH1.0 and pH4.5 buffer respectively,detect the absorbance at the wavelength of 5 10 nm and 700 nm Anthocyanin content was calculate with Fuleki T formula.Results:The detection results indicated that the anthocyan of three batch of crataegi fructus was 52.18、57.34、49.25 mg/g.Conclusion:pH-differential method can be used in quantitative analysis of anthocyan in crataegi fructus,and the impuritys interference can be eliminated.
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