Effects of Chromatin Opening Status on Pathways Associated with Esophageal Cancer and Analysis of Key Genes
Objective:To explore the effect of chromatin openness status on esophageal cancer-related pathways and to analyze the key genes.Methods:Based on the chromatin openness high-throughput sequencing(ATAC-seq)data and transcriptome sequen-cing(RNA-seq)data downloaded from the TCGA database,both sets of data were annotated using R4.2.1,and KEGG pathway enrichment analysis with GO functional enrichment analysis was performed.Differential analysis was performed on RNA-seq data of the esophageal cancer,ARL5B and RUNX1 genes that were highly correlated with chromatin open status were screened,and the KM survival curve was plotted.Univariate and multivariate COX analysis was performed for ARL5B and RUNX1.Results:The percenta-ges of ATAC-seq data in esophageal cancer with peaks≤1 kb,1～2 kb,and 2～3 kb away from the transcription start site were 32.59%,6.00%,4.41%,respectively,and peaks located in the distal intergenic region accounted for 27.15%,which was consistent with the distribution of chromatin open regions.The vast majority of the chromatin open region peaks existed near transcription start sites,which was also consistent with the characteristics of chromatin openness.The results of KEGG and GO functional analyses showed that the annotated genes were significantly enriched in the Rap1 signaling pathway,Hippo signaling pathway,ErbB signaling pathway,mTOR signaling pathway,etc.The prognosis of esophageal cancer patients with high expression of ARL5B was poorer(P<0.05).The prognosis of esophageal cancer patients with high expression of RUNX1 was better(P<0.05).Conclusion:Chro-matin openness plays an important role in regulating the functions related to the occurrence and development of esophageal cancer,and ARL5B and RUNX1 may become therapeutic targets and prognostic indicators for esophageal cancer.
NETL
NSTL
万方数据
维普
