Study on Dynamic Changes of Gastric Mucosal Flora during the Occurrence of Gastric Cancer
Objective:To explore the dynamic changes of gastric mucosal flora in the occurrence of gastric cancer(GC).Methods:8 patients with superficial gastritis(SG),9 patients with atrophic gastritis(AG),12 patients with intestinal metaplasia and dysplasia(IM/DYS),and 14 patients with GC were collected.High-throughput sequencing of the 16S rDNA gene was used to detect the gastric mucosal flora of GC patients and patients with gastric precancerous lesions.The functional differences of microbio-ta were predicted by phylogenetic investigation of communities by reconstruction of unobserved states(PICRUSt).Results:At the phylum level,the composition of the top five most abundant bacterial flora was the same among the four groups,but the proportions were different.During the process from SG to GC,the abundance of Proteobacteria and Actinobacteria showed a decreasing trend,while that of Firmicutes and Bacteroidetes showed an increasing trend.At the genus level,the abundance of Prevotella and Lactoba-cillus increased from SG to GC,and the abundance of Helicobacter reached the lowest in the GC group.Meanwhile,Lactobacillus,Dealisteria,Macrococcus,Micromononas,and Condensates were significantly enriched in the GC group.LefSe analysis showed that Bacteria was the dominant genus in the SG group,while Rubellimicrobium,Rhodobacteraceae,and Arcobacter were the dominant genera in the IM/DYS group.Coriobacteriaceae was the dominant genus in the GC group,and no significantly different metabolic pathways were enriched in KEGG.Conclusion:There are significant differences in the community and diversity of gastric mucosa flora between GC patients and precancerous lesions.
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