Isolation,Identification,and Enzymatic Characteristics of a Bacillus Strain
Objective:To isolate strains with keratinase and cellulase activities,and to identify the obtained strains.Methods:Soil samples were taken from the ground of a free-range chicken farm in a bamboo forest.The strains capable of de-grading both keratin and cellulose were selected by using feather powder medium and sodium carboxymethyl cellulose medium,respectively.16S rDNA sequence analysis,physiological and biochemical tests,and enzyme activity measurements were car-ried out.Results:A strain was isolated,and numbered as WYM41.Its 16S rDNA sequence was compared,and the similarity with Bacillus thuringiensis was 98.84％.Through morphological observation of bacteria and identification of physiological and biochemical characteristics,the strain was preliminarily identified as Bacillus genus.The degradation experiment showed that strain WYM41 had strong degradation ability on feathers,carboxymethyl cellulose sodium,and filter paper.Enzyme activity measurement showed that the enzyme activity was(559.78±43.831)U per 100 mL fermentation broth.Conclusion:The strain WYM41 has typical characteristics of Bacillus and can produce keratinase and cellulase,which has a promising applica-tion in the development and utilization of microbial resources.
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