Effect of UCH-L3 Gene Silencing on the Biological Function of Human Breast Cancer Cell MCF7
Objective:To investigate the effect of silencing UCH-L3 gene on the biological function of non-triple-negative breast cancer cell strain MCF7.Methods:The UCH-L3 silencing expression model was constructed by transfecting MCF7 cells with UCH-L3 targeted siRNA.Real-time quantitative PCR and Western blot were used to detect the expression levels of mRNA and protein,respectively.Cell proliferation and clonogenesis were measured by CCK8 test and clone formation assay.The cell invasion ability was evaluated by the transwell method.Results:After silencing processing by 3 different siRNAs,UCH-L3-001 showed the most obvious silencing effect,the mRNA expression level decreased to 24.7%of the control group,and the protein expression level de-creased to 32.0%of the control group.Compared with the black control group,UCH-L3-001 siRNA silencing,decreased the pro-liferation rate of MCF7 cells by 17.5%,the cloning capacity by 57.8%,the number of migrating clones by 55.0%,and increased the apoptosis rate by 61.6%.Conclusion:Silencing UCH-L3 can effectively inhibit the proliferation and clonogenesis of MCF7 cells,and reduce the invasion ability and promote cell apoptosis,which is expected to be a new strategy for the treatment of non-tri-ple-negative breast cancer.
UCH-L3gene silencingnon triple negative breast cancerproliferationinvasion
万方数据
维普
